Basic Information

Dataset GSE72156
Status 2016-02-17
Species Homo sapiens
Source NCBI GEO
Subcellular Localization nuclear|cytoplasm
PMID 27754875
Title Global analysis of pre-mRNA subcellular localization upon splicing inhibition by spliceostatin A
Citation Yoshimoto R, Kaida D, Furuno M, Burroughs AM et al. Global analysis of pre-mRNA subcellular localization following splicing inhibition by spliceostatin A. RNA 2017 Jan;23(1):47-57.
Experimental Design nuclear and cytoplasmic fractions were isolated using PARIS kit (Life technologies). HeLa cells were collected, and then nuclear and cytoplasmic RNAs were purified using PARIS kit (Life technologies) according to the manufacturer’s instruction.

Sample Information

BioProject Sample Name Localization Treatment Source Label
PRJNA293216 GSM1856262 nuclear MeOH HeLa S3 NM1
PRJNA293216 GSM1856263 nuclear SSA HeLa S3 NS1
PRJNA293216 GSM1856264 cytoplasm MeOH HeLa S3 CM1
PRJNA293216 GSM1856265 cytoplasm SSA HeLa S3 CS1
PRJNA293216 GSM1856266 nuclear MeOH HeLa S3 NM2
PRJNA293216 GSM1856267 nuclear SSA HeLa S3 NS2
PRJNA293216 GSM1856268 cytoplasm MeOH HeLa S3 CM2
PRJNA293216 GSM1856269 cytoplasm SSA HeLa S3 CS2
PRJNA293216 GSM1856270 nuclear MeOH HeLa S3 NM3
PRJNA293216 GSM1856271 nuclear SSA HeLa S3 NS3
PRJNA293216 GSM1856272 cytoplasm MeOH HeLa S3 CM3
PRJNA293216 GSM1856273 cytoplasm SSA HeLa S3 CS3

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Localization Differential Analysis

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Dynamic RNA Localization

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Specific Enrichment Analysis

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Specific RNA Ratio

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