Basic Information
| Dataset | GSE66230 |
|---|---|
| Status | 2015-10-13 |
| Species | Mus musculus |
| Source | NCBI GEO |
| Subcellular Localization | axonal|somatodendritic |
| PMID | 26464439 |
| Title | Subcellular whole transcriptome profiling reveals the RNA composition of motor axons |
| Citation | Briese M, Saal L, Appenzeller S, Moradi M et al. Whole transcriptome profiling reveals the RNA content of motor axons. Nucleic Acids Res 2016 Feb 29;44(4):e33. |
| Experimental Design | Spinal cords were isolated from E12.5 CD-1 mouse embryos and motoneurons derived from them were cultured as previously described (Wiese et al. 2010). Briefly, lumbar spinal cord tissues were dissected and motoneurons enriched using p75NTR antibody panning. Microfluidic chambers (Xona Microfluidics, SND 150) were precoated with polyornithine and laminin-111 (Life Technologies) and 1,000,000 motoneurons were directly plated in the somatodendritic main channel of a microfluidic chamber. Motoneurons were grown in neurobasal medium (Invitrogen) containing 500 µM GlutaMAX (Invitrogen), 2% horse serum (Linaris), 2% B27 supplement (Invitrogen) for 7 days at 37°C and 5% CO2. CNTF (5 ng/ml) was applied to both the somatodendritic and the axonal compartment. To induce axon growth through the microchannels of the chamber BDNF (20 ng/ml) was added to the axonal compartment. Culture medium was exchanged on day 1 and then every second day. |
Sample Information
| BioProject | Sample Name | Localization | Treatment | Develop Stage | Source | Label |
|---|---|---|---|---|---|---|
| PRJNA276148 | GSM1617470 | axonal compartment | wild-type | None | Primary mouse motoneuron culture | wt_ax_1 |
| PRJNA276148 | GSM1617471 | axonal compartment | wild-type | None | Primary mouse motoneuron culture | wt_ax_2 |
| PRJNA276148 | GSM1617472 | axonal compartment | wild-type | None | Primary mouse motoneuron culture | wt_ax_3 |
| PRJNA276148 | GSM1617473 | axonal compartment | wild-type | None | Primary mouse motoneuron culture | wt_ax_4 |
| PRJNA276148 | GSM1617474 | axonal compartment | wild-type | None | Primary mouse motoneuron culture | wt_ax_5 |
| PRJNA276148 | GSM1617475 | somatodendritic compartment | wild-type | None | Primary mouse motoneuron culture | wt_zk_1 |
| PRJNA276148 | GSM1617476 | somatodendritic compartment | wild-type | None | Primary mouse motoneuron culture | wt_zk_2 |
| PRJNA276148 | GSM1617477 | somatodendritic compartment | wild-type | None | Primary mouse motoneuron culture | wt_zk_3 |
| PRJNA276148 | GSM1617478 | somatodendritic compartment | wild-type | None | Primary mouse motoneuron culture | wt_zk_4 |
| PRJNA276148 | GSM1617479 | somatodendritic compartment | wild-type | None | Primary mouse motoneuron culture | wt_zk_5 |
| PRJNA276148 | GSM1841537 | somatodendritic compartment | sd | E12.5 | Primary mouse motoneuron culture | sd_1 |
| PRJNA276148 | GSM1841540 | somatodendritic compartment | sd | E12.5 | Primary mouse motoneuron culture | sd_2 |
| PRJNA276148 | GSM1841542 | somatodendritic compartment | sd | E12.5 | Primary mouse motoneuron culture | sd_3 |
| PRJNA276148 | GSM1841544 | somatodendritic compartment | sd.dil | E12.5 | Primary mouse motoneuron culture | sd_dil_1 |
| PRJNA276148 | GSM1841547 | somatodendritic compartment | sd.dil | E12.5 | Primary mouse motoneuron culture | sd_dil_2 |
| PRJNA276148 | GSM1841549 | somatodendritic compartment | sd.dil | E12.5 | Primary mouse motoneuron culture | sd_dil_3 |
Subcellular Localization Number
RNA Type:
Condition:
Localization Differential Analysis
RNA Type:
Localization:
Condition:
Method:
Specific Subcellular Localization
RNA Type:
Method:
Dynamic RNA Localization
RNA Type:
Method:
Specific Enrichment Analysis
RNA Type:
Localization:
Condition:
Method:
Enrichment:
Specific RNA Ratio
RNA Type:
RNA Symbol:
TPM:
