Basic Information

Dataset GSE183148
Status 2021-09-07
Species Homo sapiens
Source NCBI GEO
Subcellular Localization cytosol|ER
PMID 34615539
Title RNA-seq of ER-targeted and cytosolic mRNAs with control or CNOT1-targeting siRNA treatment in HEK293 cells
Citation Gillen SL, Giacomelli C, Hodge K, Zanivan S et al. Differential regulation of mRNA fate by the human Ccr4-Not complex is driven by coding sequence composition and mRNA localization. Genome Biol 2021 Oct 6;22(1):284.
Experimental Design HEK293 cells were treated with control or CNOT1-targeting siRNA for 48hrs. Cell lysates were then separated into cytosolic and ER fractions. The RNA in each fraction was sequenced.

Sample Information

BioProject Sample Name Localization Genotype Source Label
PRJNA759272 GSM5552063 cytosol control siRNA HEK293 cells Rep1_siControl_cyto
PRJNA759272 GSM5552064 ER control siRNA HEK293 cells Rep1_siControl_ER
PRJNA759272 GSM5552065 cytosol control siRNA HEK293 cells Rep2_siControl_cyto
PRJNA759272 GSM5552066 ER control siRNA HEK293 cells Rep2_siControl_ER
PRJNA759272 GSM5552067 cytosol control siRNA HEK293 cells Rep3_siControl_cyto
PRJNA759272 GSM5552068 ER control siRNA HEK293 cells Rep3_siControl_ER
PRJNA759272 GSM5552069 cytosol CNOT1 siRNA HEK293 cells Rep1_siCNOT1_cyto
PRJNA759272 GSM5552070 ER CNOT1 siRNA HEK293 cells Rep1_siCNOT1_ER
PRJNA759272 GSM5552071 cytosol CNOT1 siRNA HEK293 cells Rep2_siCNOT1_cyto
PRJNA759272 GSM5552072 ER CNOT1 siRNA HEK293 cells Rep2_siCNOT1_ER
PRJNA759272 GSM5552073 cytosol CNOT1 siRNA HEK293 cells Rep3_siCNOT1_cyto
PRJNA759272 GSM5552074 ER CNOT1 siRNA HEK293 cells Rep3_siCNOT1_ER

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Dynamic RNA Localization

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Specific RNA Ratio

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