Basic Information

Dataset E-MTAB-3585
Status 2016-07-13
Species Homo sapiens
Source EBI ArrayExpress
Subcellular Localization nuclear|cytoplasm
Title Posttranscriptional mRNA expression changes in stress
Experimental Design HEK T293 cells were treated with Anisomycin or the solvent control DMSO. Cells were fractionated and cytoplasmic and nuclear RNA were isolated. pre-mRNA-Seq was performed on stressed and unstressed nuclear RNA samples. mRNA-Seq was performed on stressed and unstressed cytoplasmic RNA samples. In addition, polyadenylation sites were globally mapped by applying the 3'T-fill method on the same stressed and unstressed cytoplasmic RNA samples.

Sample Information

BioProject Sample Name Run Localization Treatment Library Type Source Label
PRJEB9446 ERS739348 ERR899353 nuclear Anisomycin standard TruSeq HEK T293 cells nuc_nucRNA-AnisoRIII
PRJEB9446 ERS739352 ERR899356 cytoplasm Anisomycin standard TruSeq HEK T293 cells cytRNA-Aniso-RIII
PRJEB9446 ERS739355 ERR899358 nuclear DMSO standard TruSeq HEK T293 cells nuc_nucRNA-DMSO--RII
PRJEB9446 ERS739345 ERR899359 nuclear DMSO standard TruSeq HEK T293 cells nuc_nucRNA-DMSO-R-I
PRJEB9446 ERS739354 ERR899360 cytoplasm DMSO standard TruSeq HEK T293 cells cytRNA-DMSO-RIII
PRJEB9446 ERS739349 ERR899361 nuclear Anisomycin standard TruSeq HEK T293 cells nuc_nucRNA-Aniso-R-I
PRJEB9446 ERS739351 ERR899364 cytoplasm Anisomycin standard TruSeq HEK T293 cells cytRNA-Aniso-R-I
PRJEB9446 ERS739346 ERR899366 cytoplasm DMSO standard TruSeq HEK T293 cells cytRNA-DMSO-R-I
PRJEB9446 ERS739350 ERR899368 nuclear Anisomycin standard TruSeq HEK T293 cells nuc_nucRNA-Aniso-RII
PRJEB9446 ERS739347 ERR899372 cytoplasm Anisomycin standard TruSeq HEK T293 cells cytRNA-Aniso-RII
PRJEB9446 ERS739353 ERR899373 nuclear DMSO standard TruSeq HEK T293 cells nuc_nucRNA-DMSO-RIII
PRJEB9446 ERS739344 ERR899375 cytoplasm DMSO standard TruSeq HEK T293 cells cytRNA-DMSO-R-II
PRJEB9446 ERS739344 ERR904418 cytoplasm DMSO 3T-fill HEK T293 cells cytRNA-DMSO-R-II
PRJEB9446 ERS739354 ERR904419 cytoplasm DMSO 3T-fill HEK T293 cells cytRNA-DMSO-RIII
PRJEB9446 ERS739347 ERR904420 cytoplasm Anisomycin 3T-fill HEK T293 cells cytRNA-Aniso-RII
PRJEB9446 ERS739352 ERR904421 cytoplasm Anisomycin 3T-fill HEK T293 cells cytRNA-Aniso-RIII
PRJEB9446 ERS739346 ERR904422 cytoplasm DMSO 3T-fill HEK T293 cells cytRNA-DMSO-R-I
PRJEB9446 ERS739351 ERR904423 cytoplasm Anisomycin 3T-fill HEK T293 cells cytRNA-Aniso-R-I

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Specific Subcellular Localization

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Dynamic RNA Localization

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Specific Enrichment Analysis

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Specific RNA Ratio

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